Junior Members Forum

Objective: The molecular changes associated with transition of melanoma cells to the invasive phenotype are poorly understood. Methods: In this study, B16-F1 melanoma cells will be transfected into a gene construct that may influence cell invasion. Expression of this gene and its coregulators will be assessed by immumohistochemistry in human archival melanoma samples, against the Breslow thickness scale, differentiation and nodal metastasis. Outcome: This study may elucidate molecular determinants of melanoma prognosis and provide novel molecular targets for therapy. S2. A study of the physical rehabilitation and psychological state of patients who sustained limb loss as a result of terrorist Objective: To benchmark the psychological state and physical rehabilitation of patients who have sustained limb loss as a result of terrorist activity in Northern Ireland and to determine their satisfaction with the period of primary prosthetic rehabilitation and the artificial limb. Methods: All patients who sustained limb loss as a result of the Troubles and were referred to our rehabilitation centre were sent a questionnaire. The main outcome measures were the SIGAM mobility grades, the General Health Questionnaire (GHQ12) and 3 screening questions for Post Traumatic Stress Disorder (PTSD). Results: 66% response rate. 52 (69%) patients felt that the period of primary prosthetic rehabilitation was adequate. 32 (54%) lower limb amputees graded themselves SIGAM C or D. 45 (60%) patients stated that they were still having significant stump pain. Significant stump pain and symptoms of PTSD were both associated with poorer mobility. 9 (56%) upper limb amputees used their prosthetic limb in a functional way. 33 (44%) patients showed psychiatric caseness on the GHQ 12 and 50 (67%) had symptoms of PTSD. Conclusions: Most patients felt that the period of physical rehabilitation had been adequate those who did not were more likely to be having ongoing psychological problems. A high percentage of patients continue to have psychological problems and stump pain both of which were associated with poorer mobility.